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Characterisation and diversity of Indian isolates of Ralstonia solanacearum causing bacterial wilt of Capsicum annuum L.

Author:
Singh, Dinesh, Chaudhary, Garima, Yadav, D. K.
Source:
Archiv für Phytopathologie und Pflanzenschutz 2018 v.51 no.5-6 pp. 267-276
ISSN:
1477-2906
Subject:
Capsicum annuum, Ralstonia solanacearum, bacteria, bacterial wilt, genes, genetic variation, hot peppers, multilocus sequence typing, pathogenicity, phylotype, sweet peppers, India
Abstract:
Genetic diversity of 13 isolates of Ralstonia solanacearum causing bacterial wilt in hot pepper and bell pepper (Capsicum annuum L.) from 6 states of India was assessed. All isolates of R. solanacearum belonged to biovar 3, race 1 and phylotype I. These isolates consisted of 4 distinct DNA types at 75% similarity coefficient using ERIC, BOX and REP-PCRs techniques. Multilocus sequence analysis of hrpB, fliC and egl genes of 6 isolates of R. solanacearum along with 2 out group bacteria was done and they showed high level of variability within these three regions of the genome involving in pathogenicity.
Agid:
6092968