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The effects of serial skin testing with purified protein derivative on the level and quality of antibodies to complex and defined antigens in Mycobacterium bovis-infected cattle

W. Ray Waters, Mitchell V. Palmer, Molly R. Stafne, Kristin E. Bass, Mayara F. Maggioli, Tyler C. Thacker, Rick Linscott, John C. Lawrence, Jeffrey T. Nelson, Javan Esfandiari, Rena Greenwald, Konstantin P. Lyashchenko
Clinical and vaccine immunology 2015 v.22 no.6 pp. 641-649
Mycobacterium bovis, antibodies, antibody affinity, antibody formation, bacterial antigens, bovine tuberculosis, cattle, immunoglobulin G, immunoglobulin M, proteinases, skin tests
Several serologic tests designed to detect antibodies to immunodominant Mycobacterium bovis antigens have recently emerged as ancillary tests for the detection of bovine tuberculosis in cattle, particularly when applied after injection of purified protein derivative (PPD) for skin test that significantly boosts M. bovis-specific antibody responses. Present findings demonstrate the onset and duration of boosted antibody responses after injection of M. bovis PPD for the caudal fold test (CFT) and M. avium and M. bovis PPDs for the comparative cervical test (CCT) applied in series in cattle experimentally-infected with M. bovis. While application of skin tests boosted the response to certain antigens (i.e., MPB83 and MPB70), it did not impact the response to other antigens (e.g., ESAT-6, CFP10, MPB59, and MPB64). Administration of the CCT 105 days after CFT resulted in an even greater secondary boost in antibody responses to MPB83/70 and a proteinase K-digested whole cell sonicate of M. bovis (WCS-PK). Both IgM and IgG contributed to the initial boost in the MPB83/70-specific antibody response after CFT. The secondary boost after CCT was primarily due to increased IgG. Also, the avidity of antibody to MPB83/70 increased after CCT in M. bovis-infected cattle. The avidity of antibody to the WCS-PK antigen increased in the interval between CFT and CCT, but did not increase further after CCT. Together, these findings demonstrate that administration of PPDs for skin test results in additive enhancement (i.e., when the CFT and CCT is applied in series), both qualitative and quantitative, of MPB83/70-specific antibody responses.