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Protein-tannic acid multilayer films: A multifunctional material for microencapsulation of food-derived bioactives

Author:
Lau, Hooi Hong, Murney, Regan, Yakovlev, Nikolai L., Novoselova, Marina V., Lim, Su Hui, Roy, Nicole, Singh, Harjinder, Sukhorukov, Gleb B., Haigh, Brendan, Kiryukhin, Maxim V.
Source:
Journal of colloid and interface science 2017 v.505 pp. 332-340
ISSN:
0021-9797
Subject:
adhesion, beta-lactoglobulin, bioactive compounds, bovine serum albumin, digestion, functional foods, gastric juice, human cell lines, humans, immunoglobulin G, ingredients, microencapsulation, stomach
Abstract:
The benefits of various functional foods are often negated by stomach digestion and poor targeting to the lower gastrointestinal tract. Layer-by-Layer assembled protein-tannic acid (TA) films are suggested as a prospective material for microencapsulation of food-derived bioactive compounds. Bovine serum albumin (BSA)-TA and pepsin-TA films demonstrate linear growth of 2.8±0.1 and 4.2±0.1nm per bi-layer, correspondingly, as shown by ellipsometry. Both multilayer films are stable in simulated gastric fluid but degrade in simulated intestinal fluid. Their corresponding degradation constants are 0.026±0.006 and 0.347±0.005nm⁻¹min⁻¹. Milk proteins possessing enhanced adhesion to human intestinal surface, Immunoglobulin G (IgG) and β-Lactoglobulin (BLG), are explored to tailor targeting function to BSA-TA multilayer film. BLG does not adsorb onto the multilayer while IgG is successfully incorporated. Microcapsules prepared from the multilayer demonstrate 2.7 and 6.3 times higher adhesion to Caco-2 cells when IgG is introduced as an intermediate and the terminal layer, correspondingly. This developed material has a great potential for oral delivery of numerous active food-derived ingredients.
Agid:
6094163