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ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation

Author:
Bergam, Ptissam, Reisecker, Johannes M., Rakvács, Zsófia, Kucsma, Nóra, Raposo, Graça, Szakacs, Gergely, van Niel, Guillaume
Source:
Journal of molecular biology 2018 v.430 no.20 pp. 3802-3818
ISSN:
0022-2836
Subject:
amyloid, electron microscopy, epithelium, etiology, gene overexpression, genes, human cell lines, humans, image analysis, inheritance (genetics), knockout mutants, lysosomes, melanogenesis, mutation, pigmentation, small interfering RNA
Abstract:
Genetically inheritable pigmentation defects provide a unique opportunity to reveal the function of proteins contributing to melanogenesis. Dyschromatosis universalis hereditaria (DUH) is a rare pigmentary genodermatosis associated with mutations in the ABCB6 gene. Here we use optical and electron microscopy imaging combined with biochemical tools to investigate the localization and function of ABCB6 in pigment cells. We show that ABCB6 localizes to the membrane of early melanosomes and lysosomes of the human melanocytic cell line MNT-1. Depletion of ABCB6 by siRNA impaired PMEL amyloidogenesis in early melanosomes and induced aberrant accumulation of multilamellar aggregates in pigmented melanosomes. PMEL fibril formation and normal maturation of pigmented melanosomes could be restored by the overexpression of wild-type ABCB6 but not by variants containing an inactivating catalytic mutation (K629M) or the G579E DUH mutation. In line with the impairment of PMEL matrix formation in the absence of ABCB6, morphological analysis of the retinal pigment epithelium of ABCB6 knockout mice revealed a significant decrease of melanosome numbers. Our study extends the localization of ABCB6 to melanosomes, suggesting a potential link between the function of ABCB6 and the etiology of DUH to amyloid formation in pigment cells.
Agid:
6095104