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Comparison of bacterial diversity profiles and microbial safety assessment of salami, Chinese dry-cured sausage and Chinese smoked-cured sausage by high-throughput sequencing

Author:
Wang, Xinhui, Zhang, Yalin, Ren, Hongyang, Zhan, Yi
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.90 pp. 108-115
ISSN:
0023-6438
Subject:
Brochothrix, Enterococcus, Lactobacillus, Lactococcus, Pediococcus, Photobacterium, Pseudomonas, Staphylococcus, Weissella, bacterial communities, cured meats, fermentation, fermented sausages, high-throughput nucleotide sequencing, lactic acid bacteria, meat, microbiological quality, safety assessment, salami, spoilage bacteria
Abstract:
The bacterial communities of salami, Chinese dry-cured sausage and Chinese smoked-cured sausage were compared by high-throughput sequencing technology. The bacterial communities of Chinese dry-cured sausage and Chinese smoked-cured sausage were very different from that of salami, and the bacterial distribution was more abundant than that of salami. The highest number of operational taxonomic units (OTUs) was found in salami for the genus Staphylococcus spp., accounting for 97.45%. Accordingly, Staphylococcus spp. and, and Lactic acid bacteria (LAB), including Lactobacillus spp., Weissella spp., Pediococcus spp., and Lactococcus spp. were the main bacteria both in Chinese dry-cured sausage and Chinese smoked-cured sausage. Moreover, the salami has a microbial safety with trace Enterococcus spp. (0.03%) population. In contrast, a larger Enterococcus spp. population with relative abundance of 1.16% and 2.99% was observed in Chinese dry-cured sausage and Chinese smoked-cured sausage, respectively. Apart from Enterococcus spp., several meat spoilage bacteria, namely, Photobacterium spp., Brochothrix spp. and Pseudomonas spp. were checked in Chinese-style sausages. The results revealed that the Chinese-style sausages have a poor hygienic quality, probably due to spontaneous fermentation. This study presents that high-throughput sequencing is a useful tool to evaluate bacterial diversity and monitor microbial quality in fermented sausages.
Agid:
6095446