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Chronic brain toxicity response of juvenile Chinese rare minnows (Gobiocypris rarus) to the neonicotinoid insecticides imidacloprid and nitenpyram

Author:
Tian, Xue, Yang, Wenjie, Wang, Dong, Zhao, Yue, Yao, Ruihua, Ma, Lekuan, Ge, Chazhong, Li, Xiaoliang, Huang, Zeyu, He, Li, Jiao, Wentao, Lin, Aijun
Source:
Chemosphere 2018 v.210 pp. 1006-1012
ISSN:
0045-6535
Subject:
Gobiocypris rarus, acetylcholinesterase, adverse effects, brain, catalase, chronic exposure, glutathione, imidacloprid, juveniles, malondialdehyde, messenger RNA, minnows, nitenpyram, oxidative stress, superoxide dismutase, toxicity
Abstract:
Imidacloprid and nitenpyram are widely used neonicotinoid pesticides worldwide and were observed to adversely affect non-target aquatic organisms. In this study, the toxic effect of imidacloprid and nitenpyram on the brain of juvenile Chinese rare minnows (Gobiocypris rarus) was investigated by determining the oxidative stress, 8-hydroxy-2-deoxyguanosine (8-OHdG) content and acetylcholinesterase (AChE) activity. The superoxide dismutase (SOD) activities did not significantly change after long-term exposure to imidacloprid and nitenpyram. A noticeable increase of catalase (CAT) activities was observed on the brain tissues under 0.1 mg/L imidacloprid and under all nitenpyram treatments (p < 0.05). The malondialdehyde (MDA) content increased markedly under 2.0 mg/L imidacloprid and 0.1 mg/L nitenpyram treatments (p < 0.05). The glutathione (GSH) content in the brain significantly increased under 0.5 and 2.0 mg/L imidacloprid (p < 0.05). A significant decrease was observed in the mRNA levels of Cu/Zn-sod under 2.0 mg/L imidacloprid and those of cat under 0.1 and 0.5 mg/L nitenpyram (p < 0.05). The mRNA levels of gpx1 clearly decreased under 2.0 mg/L imidacloprid and under 0.1 mg/L nitenpyram (p < 0.05). The treatments of 0.1 and 0.5 mg/L nitenpyram decreased cat expression levels markedly (p < 0.05). 2.0 mg/L imidacloprid raised the 8-OHdG content. The AChE activities increased markedly under 0.5 and 2.0 mg/L imidacloprid while clearly decreasing under 2.0 mg/L nitenpyram (p < 0.05). Therefore, our results indicate that imidacloprid and nitenpyram might cause adverse effects on juvenile Chinese rare minnows brain. Notably, imidacloprid had greater impacts on juvenile rare minnows compared to nitenpyram.
Agid:
6098068