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Protection of probiotic bacteria in a synbiotic matrix following aerobic storage at 4 °C
- Chaluvadi, S., Hotchkiss Jr., A.T., Call, J.E., Luchansky, J.B., Phillips, J.G., Liu, LS., Yam, K.L.
- Beneficial microbes 2012 v.3 no.3 pp. 175
- Bifidobacterium breve, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus reuteri, acids, aerobic conditions, bacteria, calcium, cold storage, crosslinking, dietary supplements, fructooligosaccharides, health promotion, inulin, probiotics, shelf life, storage conditions
- The survival of single strains of <i>Bifidobacterium breve, Bifidobacterium longum, Lactobacillus acidophilus,</i> and <i>Lactobacillus reuteri</i> was investigated in synbiotics that included 10 mg/ml of fructo-oligosaccharides, inulin and pectic-oligosaccharides in an alginate matrix under refrigerated (4 °C) aerobic storage conditions. When the matrices were cross-linked with calcium (45 mM), 10<sup>2</sup>-10<sup>3</sup> cfu/ml of <i>L. acidophilus</i> and <i>L. reuteri</i>, and 0-10<sup>3</sup> cfu/ml of <i>B. breve</i> and <i>B. longum</i> survived refrigerated aerobic storage for 28 days. Following refrigerated storage, acetic (3-9 mM), butyric (0-2 mM), propionic (5-16 mM) and lactic acids (1-48 mM) were produced during the growth of probiotics in BHI broth at 37 °C, suggesting their metabolic activity after storage was stressed. When calcium cross-linking was not used in synbiotics, the matrix remained more gel-like after inoculation when compared to the calcium cross-linked matrix. At least 10<sup>7</sup> cfu/ml of probiotic bacteria survived after 21 days of storage within these gel-like alginate matrices. Significantly higher levels of <i>B. breve</i>, <i>L. acidophilus</i> and <i>L. reuteri</i> were obtained from the synbiotic matrices supplemented with fructo-oligosaccharides, inulin and pectic-oligosaccharides compared to alginate alone. <i>B. longum</i> survival was the same (~7 logs) in all gel-like synbiotic matrices. These results show that synbiotics protected probiotic bacteria and extended their shelf-life under refrigerated aerobic conditions. Synbiotics represent a viable delivery vehicle for health-promoting bacteria.