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Comparison of the Purelyse® – IS6110 nested PCR with the Xpert® MTB/RIF test in clinical samples with suspected tuberculosis

Author:
Lemus-Minor, Carlos German, Ovalle-Marroqui, Diego Fernando, Vazquez-Jimenez, J. Gustavo, Reales-Agüero, Diana Laura, Sepulveda-Alcantara, Perla Michelle, Rodriguez-Sánchez, Jesús René, Diaz-Molina, Raúl, Machado-Contreras, Jesús René
Source:
Journal of microbiological methods 2018 v.152 pp. 48-51
ISSN:
0167-7012
Subject:
DNA, Mycobacterium tuberculosis complex, diagnostic techniques, mortality, patients, polymerase chain reaction, rifampicin, tuberculosis
Abstract:
Tuberculosis (TB) has a high incidence, prevalence and mortality in the world. Due to its high level of transmission and long-term pharmacological treatment, it is important to have sensitive and specific diagnostic tests. Recently, the PureLyse® system, which is a novel DNA extraction method, was proposed to be an important tool for molecular diagnosis of TB. Here, we compare the PureLyse® system followed by an IS6110 nested PCR (PureLyse® – IS6110 nested PCR) with the Xpert® MTB/RIF test for Mycobacterium tuberculosis complex (MTBC) identification in 40 clinical samples. Among the 40 samples, 26 samples were positive and 14 negative for the Xpert® MTB/RIF test as well as for the PureLyse® - IS6110 nested PCR. According to the Xpert® MTB/RIF test, positive samples presented different bacillary concentrations from “High” to “Very low” and rifampin resistance was observed in 5 samples. The concordance of both molecular methods makes the PureLyse® - IS6110 nested PCR suitable for MTBC detection in patients for low-income resources.
Agid:
6101471