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Comparative analysis of transcriptome in two wheat genotypes with contrasting levels of drought tolerance

Kumar, Jitendra, Gunapati, Samatha, Kianian, Shahryar F., Singh, Sudhir P.
Protoplasma 2018 v.255 no.5 pp. 1487-1504
Triticum aestivum, drought, drought tolerance, embryogenesis, enzymes, gene expression regulation, genotype, hormone metabolism, leaves, nucleic acids, plasma membrane, protein synthesis, regulator genes, stress response, tissues, transcription (genetics), transcriptome, transcriptomics, wheat, wilting
Drought tolerance is a complex trait that is governed by multiple genes. The study presents differential transcriptome analysis between drought-tolerant (Triticum aestivum Cv. C306) and drought-sensitive (Triticum aestivum Cv. WL711) genotypes, using Affymetrix GeneChip® Wheat Genome Array. Both genotypes exhibited diverse global transcriptional responses under control and drought conditions. Pathway analysis suggested significant induction or repression of genes involved in secondary metabolism, nucleic acid synthesis, protein synthesis, and transport in C306, as compared to WL711. Significant up- and downregulation of transcripts for enzymes, hormone metabolism, and stress response pathways were observed in C306 under drought. The elevated expression of plasma membrane intrinsic protein 1 and downregulation of late embryogenesis abundant in the leaf tissues could play an important role in delayed wilting in C306. The other regulatory genes such as MT, FT, AP2, SKP1, ABA2, ARF6, WRKY6, AOS, and LOX2 are involved in defense response in C306 genotype. Additionally, transcripts with unknown functions were identified as differentially expressed, which could participate in drought responses.