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The atherogenic actions of LPC on vascular smooth muscle cells and its LPA receptor mediated mechanism

Bao, Liang, Qi, Jingwei, Wang, Yue-wu, Xi, Qilemuge, Tserennadmid, Tseveensuren, Zhao, Peng-fei, Qi, Jerry, Damirin, Alatangaole
Biochemical and biophysical research communications 2018 v.503 no.3 pp. 1911-1918
animal models, antagonists, aorta, cardiovascular diseases, drugs, genes, humans, low density lipoprotein, lysophosphatidylcholine, lysophospholipase, messenger RNA, mitogen-activated protein kinase, monocytes, myocytes, oxidation, pertussis toxin, rabbits, signal transduction, small interfering RNA, smooth muscle, therapeutics
Lysophosphatidylcholine (LPC) is a bioactive lipid constituent of oxidized low density lipoprotein (ox-LDL). It regulates various cellular functions, including migration of circulating monocytes, expression of endothelial adhesion molecules, proliferation and migration of vascular smooth muscle cells (VSMCs). LPC can also be hydrolyzed into lysophosphatidic acid (LPA) by autotaxin (ATX) which possesses lysophospholipase D (lyso-PLD) activity. The aim of this study was to explore the effects of LPC on proliferation and migration of human artery smooth muscle cells (HASMCs) and the involvement of LPC-ATX-LPA pathway in these processes. In vitro, we found that LPC and LPA stimulated HASMCs proliferation and migration. Knockdown of LPA1 by siRNA and inhibit Gi protein with pertussis toxin (PTX) showed the contrary results. Silencing of LPC receptor genes did not significantly affect the LPC induced proliferation and migration. We detected the higher expressed mRNA and protein of ATX in HASMCs, and measured lyso-PLD activity. In atherosclerotic rabbit model, we observed high LPC level and high lyso-D activity in blood, and high expression of LPA1 in aorta walls. We also found that neointima appeared to be thickened and mRNA expressions of LPA1 appeared to be increased. These results revealed that LPC was converted into LPA by ATX to induce the proliferation and migration in HASMCs through LPA1/Gi/o/MAP Kinase signaling pathway. Our research suggested that LPC-ATX-LPA system contributed to the atherogenic action induced by ox-LDL. LPA1 antagonist may be considered as a potential therapeutic and preventative drug for cardiovascular disease.