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Structural characterization and antioxidant activities of a novel polysaccharide fraction from the fruiting bodies of Craterellus cornucopioides

Author:
Yang, Wei-Wei, Wang, Li-Ming, Gong, Li-Li, Lu, Yong-Ming, Pan, Wen-Juan, Wang, Ya, Zhang, Wen-Na, Chen, Yan
Source:
International journal of biological macromolecules 2018 v.117 pp. 473-482
ISSN:
0141-8130
Subject:
2,2-diphenyl-1-picrylhydrazyl, Craterellus cornucopioides, antioxidant activity, arabinose, catalase, erythrocytes, free radicals, fructose, fruiting bodies, glucose, glutathione peroxidase, hemolysis, malondialdehyde, mannose, mice, molecular weight, oxidative stress, polysaccharides, reactive oxygen species, xylose
Abstract:
A new polysaccharide fraction (CCPP-1) was obtained from Craterellus cornucopioides. CCPP-1 had an average molecular weight of 9.2 × 10⁵ Da, which was mainly composed of mannose, glucose, xylose, arabinose, fructose in molar ratio of 0.7:0.05:0.18:1:0.05. Results of structural characterization revealed that the dominant linkage types of CCPP-1 were →3, 6)-Manp(1→, T-Araf, →4, 6)-Manp (1→, →5)-Araf (1→ and →3)-Araf (1→. Interesting, in vitro antioxidant activities assays showed that CCPP-1 possessed strong scavenging abilities on DPPH and ABTS radicals. The oxidative hemolysis induced by AAPH in mice erythrocytes was effectively reversed by incubation with CCPP-1. CCPP-1 significantly prevented AAPH-induced intracellular reactive oxygen species (ROS) generation. Moreover, CCPP-1 could significantly restore AAPH-induced increase of intracellular antioxidant enzyme glutathione peroxidase (GPx) and catalase (CAT) activities to normal level, as well as inhibit intracellular malondialdehyde (MDA) formation. Therefore, CCPP-1 could protect against AAPH-induced oxidative-stress in erythrocytes, which would be explored as naturally potential antioxidant agent applied in food and cosmetic fields.
Agid:
6103957