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Production of a monoclonal antibody against Porcine circovirus type 3 cap protein

Li, Xiangdong, Bai, Yilin, Zhang, Haiyang, Zheng, Dingding, Wang, Tongyan, Wang, Yanwei, Deng, Junhua, Sun, Zhe, Tian, Kegong
Journal of virological methods 2018 v.261 pp. 10-13
Porcine circovirus, antiserum, cell lines, immunoassays, immunohistochemistry, monoclonal antibodies, pathogenesis, staining, structural proteins, swine, viruses
Porcine cirvovirus type 3 (PCV3) is a newly emerging swine virus with worldwide distribution. The pathogenesis of PCV3 remains unknown due to failures in propagating and isolating the virus on successive cell lines. The virus cap protein is the only structural protein of PCV3 and no monoclonal antibodies against it are available. Although antisera are available from PCV3-infected pigs, they are not suitable for immunoassays such as immunohistochemical staining of infected tissues due to high non-specific background. Developing monoclonal antibodies against the cap protein is indispensable for elucidating PCV3 biological properties. In this study, a monoclonal antibody (mAb 1H1) with a titer of 1/25,600 that recognized the PCV3 cap protein was developed. Western-blot results showed that mAb 1H1 reacted strongly with the recombinant PCV3 cap protein preparation but not with the PCV2 cap protein. MAb 1H1 was also applied successfully for the detection of the cap protein in PCV3-infected pig tissues using immunochemistry staining. In conclusion, mAb 1H1 has significant potential uses in PCV3 diagnosis as well as the study of PCV3 biology.