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The impact of heating on the unfolding and polymerization process of frozen-stored gluten
- Wang, Pei, Zou, Min, Tian, Mengqi, Gu, Zhenxin, Yang, Runqiang
- Food hydrocolloids 2018 v.85 pp. 195-203
- dough quality, freezing, frozen dough, frozen storage, gliadin, gluten, glutenins, heat, heat treatment, hydrocolloids, hydrophobicity, microstructure, molecular weight, polymerization, temperature
- Gluten deterioration is the main factor for the degraded quality of frozen dough. Previous studies comprehensively elucidated the degradation mechanism of gluten during frozen storage, however, the subsequent polymerization process of frozen-stored gluten remains largely unelucidated. The current study comparatively investigated the effects of heating on the unfolding and polymerization process of fresh and frozen-stored gluten from molecular weight, subunit composition, non-covalent interactions, secondary structure, surface hydrophobicity and microstructure. The results showed that frozen storage degraded the polymerization of gluten during heating by weakening the polymerization ability of both gliadin and glutenin. Glutenin monomers were more sensitive, while γ-gliadins were less sensitive to polymerize upon heating for frozen gluten as compared with that of the fresh gluten. Frozen storage could impede the unfolding process at the initial heating stage for frozen gluten: the ordered α-helices in frozen gluten was significantly higher than that of fresh gluten during heat treatment, and the surface hydrophobic groups were more buried than fresh gluten. The microstructure analysis suggested that the ruptured frozen gluten network was more sensitive to shrink than the fresh gluten network during the initial heating at 50 °C and further formed denser and thicker pore walls when the temperature exceeded 70 °C. The results of this study may consummate the deterioration theory of frozen gluten quality from both freezing and heating stages, and thus providing a more comprehensive theoretical basis and technical support for the effective preservation of frozen dough quality.