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Immune and anti-oxidant effects of in ovo selenium proteinate on post-hatch experimental avian necrotic enteritis
- Lee, Sung Hyen, Lillehoj, Hyun S., Jang, Seung I., Jeong, Misun, Kim, Duk Kyung, Xu, Shouzhen, Lee, Seung Kyu, Kim, Jung Bong, Park, Hong Ju, Kim, Haeng Ran, Bravo, David M.
- Veterinary parasitology 2014 v.206 no.3-4 pp. 115
- Clostridium perfringens, Eimeria maxima, antibodies, antioxidants, arylesterase, blood serum, body weight, broiler chickens, catalase, catalytic activity, eggs, embryogenesis, genes, glutathione peroxidase, hydrolysis, inducible nitric oxide synthase, interleukin-1beta, interleukin-6, interleukin-8, malondialdehyde, messenger RNA, necrotic enteritis, oocysts, protein hydrolysates, selenium, soy protein, superoxide dismutase
- This study was conducted to investigate the effects of in ovo administration of selenium (Se) incorporated into hydrolyzed soybean protein (B-Taxim [BT]) on protection against experimental avian necrotic enteritis (NE). Broiler eggs were injected with either 100μl of PBS alone (BT0), or 20 or 40μg/egg of BT in PBS (BT20, BT40) at 18 days of embryogenesis. On day 14 post-hatch, the chickens were uninfected or orally infected with 1.0×104 oocysts of Eimeria maxima (E. maxima). On day 18 post-hatch, E. maxima-infected chickens were orally infected with 1.0×109CFU of Clostridium perfringens (C. perfringens). Compared with untreated and infected BT0 controls, BT20 and/or BT40 birds showed increased body weights, decreased fecal shedding of E. maxima oocysts, lower serum α-toxin and NetB levels, increased levels of serum antibodies against C. perfringens α-toxin and NetB toxin, decreased levels of serum malondialdehyde, reduced serum catalase and superoxide dismutase catalytic activities, and increased intestinal levels of gene transcripts encoding interleukin (IL)-1β, IL-6, IL-8, and peroxiredoxin-6, but decreased levels of transcripts for catalase and glutathione peroxidase. Interestingly, transcript levels for inducible nitric oxide synthase and paraoxonase/arylesterase 2 were decreased in the BT20 group and increased in the BT40 group, compared with BT0 controls. These results indicate that in ovo administration of broiler chickens with a Se-containing protein hydrolysate enhanced protection against experimental NE possibly by altering the expression of proinflammatory and anti-oxidant genes and their downstream pathways.