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Immune and anti-oxidant effects of in ovo selenium proteinate on post-hatch experimental avian necrotic enteritis

Lee, Sung Hyen, Lillehoj, Hyun S., Jang, Seung I., Jeong, Misun, Kim, Duk Kyung, Xu, Shouzhen, Lee, Seung Kyu, Kim, Jung Bong, Park, Hong Ju, Kim, Haeng Ran, Bravo, David M.
Veterinary parasitology 2014 v.206 no.3-4 pp. 115
Clostridium perfringens, Eimeria maxima, antibodies, antioxidants, arylesterase, blood serum, body weight, broiler chickens, catalase, catalytic activity, eggs, embryogenesis, genes, glutathione peroxidase, hydrolysis, inducible nitric oxide synthase, interleukin-1beta, interleukin-6, interleukin-8, malondialdehyde, messenger RNA, necrotic enteritis, oocysts, protein hydrolysates, selenium, soy protein, superoxide dismutase
This study was conducted to investigate the effects of in ovo administration of selenium (Se) incorporated into hydrolyzed soybean protein (B-Taxim [BT]) on protection against experimental avian necrotic enteritis (NE). Broiler eggs were injected with either 100μl of PBS alone (BT0), or 20 or 40μg/egg of BT in PBS (BT20, BT40) at 18 days of embryogenesis. On day 14 post-hatch, the chickens were uninfected or orally infected with 1.0×104 oocysts of Eimeria maxima (E. maxima). On day 18 post-hatch, E. maxima-infected chickens were orally infected with 1.0×109CFU of Clostridium perfringens (C. perfringens). Compared with untreated and infected BT0 controls, BT20 and/or BT40 birds showed increased body weights, decreased fecal shedding of E. maxima oocysts, lower serum α-toxin and NetB levels, increased levels of serum antibodies against C. perfringens α-toxin and NetB toxin, decreased levels of serum malondialdehyde, reduced serum catalase and superoxide dismutase catalytic activities, and increased intestinal levels of gene transcripts encoding interleukin (IL)-1β, IL-6, IL-8, and peroxiredoxin-6, but decreased levels of transcripts for catalase and glutathione peroxidase. Interestingly, transcript levels for inducible nitric oxide synthase and paraoxonase/arylesterase 2 were decreased in the BT20 group and increased in the BT40 group, compared with BT0 controls. These results indicate that in ovo administration of broiler chickens with a Se-containing protein hydrolysate enhanced protection against experimental NE possibly by altering the expression of proinflammatory and anti-oxidant genes and their downstream pathways.