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Novel in vitro method for measuring the mass fraction of bioaccessible atmospheric polycyclic aromatic hydrocarbons using simulated human lung fluids

Yu, Yingxin, Jiang, Zi'an, Zhao, Zhishen, Chong, Dan, Li, Guiying, Ma, Shengtao, Zhang, Yanan, An, Taicheng
Environmental pollution 2018 v.242 pp. 1633-1641
bioavailability, equations, health effects assessments, human health, in vitro studies, lungs, octanol-water partition coefficients, pollutants, polycyclic aromatic hydrocarbons, risk
The bioaccessibility of organic pollutants is a key factor in human health risk assessments. We developed a novel in vitro method for determining the mass fraction of bioaccessible atmospheric polycyclic aromatic hydrocarbons (PAHs) using an air-washing device containing simulated human lung fluid. The experimental parameters were optimized based on the deposition fractions (DFs) of PAHs in human lung fluids. The DFs were measured for PAHs based on the mass of compounds in the mainstream and exhaled cigarette smoke. The mass fractions of bioaccessible PAHs were measured by passing the mainstream cigarette smoke through the air-washing device, and they were calculated via a simple mass balance equation based on the PAHs in the fluid and mainstream cigarette smoke. The DFs of individual PAHs ranged from 20.5% to 78.1%, and the bioaccessible mass fractions varied between 45.5% and 99.8%. The octanol-water partition coefficients (KOW) significantly influenced both the DFs and bioaccessible mass fractions of PAHs, and the optimized in vitro method could be used to estimate the bioavailable atmospheric PAHs. This in vitro method can potentially be used to measure the mass fraction of bioaccessible atmospheric PAHs and to assess the health risk related to human exposure to airborne PAHs.