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Bioremediation of the neonicotinoid insecticide clothianidin by the white-rot fungus Phanerochaete sordida

Mori, Toshio, Wang, Jianqiao, Tanaka, Yusuke, Nagai, Kaoru, Kawagishi, Hirokazu, Hirai, Hirofumi
Journal of hazardous materials 2017 v.321 pp. 586-590
Phanerochaete sordida, bees, bioremediation, cell lines, clothianidin, culture media, cytochrome P-450, electrospray ionization mass spectrometry, humans, liquids, manganese peroxidase, metabolites, neurons, neurotoxicity, nuclear magnetic resonance spectroscopy, viability, white-rot fungi
Clothianidin (CLO) is a member of the neonicotinoid pesticides, which have been widely used worldwide over the last two decades. However, its toxicity for bees and neurological toxicity for humans are urgent problems. Here, the degradation of CLO by the white-rot fungus Phanerochaete sordida was examined in nitrogen-limited liquid medium. After incubation for 20days at 30°C, 37% of CLO was degraded in the cultures. High-resolution ESI–MS and NMR analyses of the culture supernatant identified N-(2-chlorothiazol-5-yl-methyl)-N′-methylurea (TZMU) as a metabolite of CLO degradation. The addition of cytochrome P450 inhibitors to the culture medium markedly reduced the degradation of CLO by P. sordida. And manganese peroxidase, a major ligninolytic enzyme secreted by this fungus, were not carried out CLO degradation. The effects of CLO and TZMU on the viability of the neuronal cell line Neuro2a demonstrated that P. sordida effectively degrades CLO into a metabolite that lacks neurotoxicity.