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The biological characteristics of sheep umbilical cord mesenchymal stem cells

Author:
Chen, F., Zhao, C., Zhao, Y., Li, L., Liu, S., Zhu, Z., Guan, W.
Source:
Canadian journal of veterinary research 2018 v.82 no.3 pp. 216-224
ISSN:
0830-9000
Subject:
adipocytes, chondrocytes, growth curves, mesenchymal stromal cells, osteoblasts, reverse transcriptase polymerase chain reaction, sheep, stem cells, therapeutics, tissue repair, umbilical cord, veterinary medicine
Abstract:
Although mesenchymal stem cells (MSCs) are now regarded as a promising cell resource for tissue repair and regeneration, the optimal source of MSCs has not yet been determined. The objective of this study was to provide a theoretical basis for the clinical application of umbilical cord mesenchymal stem cells (UCMSCs) in the future. Umbilical cord is an easily obtainable tissue resource, which is one reason that it has become a candidate resource for mesenchymal stem cells. In this study, we analyzed the biological characteristics of UCMSCs, such as their multiple differentiation and clone-forming ability, through morphological observation, reverse transcription polymerase chain reaction (RT-PCR), growth curve, positive rate test, and immunophenotype. Umbilical cord MSCs were successfully isolated and passaged to 29 generations. The results from RT-PCR showed that UCMSCs were positive for CD29, CD44, CD73, but negative for CD34. The expression of the stem cell marker nucleostemin and tenocyte-related markers showed similar positive results with CD44, CD73, and CD90. In addition, UCMSCs can be induced to differentiate into osteoblasts, adipocytes, or chondrocytes. Our study showed that UCMSCs not only have the ability to self-renew, but also have the potential to differentiate into multiple lineages. In general, we concluded that UCMSCs are a reliable source for use in cell therapy.
Agid:
6107670