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Application of graphene oxide-silica composite reinforced hollow fibers as a novel device for pseudo-stir bar solid phase microextraction of sulfadiazine in different matrices prior to its spectrophotometric determination

Kazemi, Elahe, Haji Shabani, Ali Mohammad, Dadfarnia, Shayessteh
Food chemistry 2017 v.221 pp. 783-789
acetic acid, azo dyes, fiber optics, graphene, honey, methanol, milk, solid phase microextraction, spectroscopy, statistical analysis, sulfadiazine, urine
This study presents a novel, simple and efficient pseudo-stir bar solid phase microextraction method for separation and preconcentration of sulfadiazine. To develop the method, a graphene oxide-silica composite reinforced hollow fiber was prepared via sol-gel technology and used as a novel device to extract sulfadiazine. The retained sulfadiazine was eluted using 180μL of methanol/acetic acid (6:4) and quantified by fiber optic linear array spectrophotometry based on the formation of its azo dye with thenoyltrifluoroacetone. Under optimized conditions, the method exhibited a linear dynamic range of 5–150μgL−1 with a detection limit of 1.5μgL−1 and an enrichment factor of 100. The relative standard deviations of 2.9% and 5.8% (n=6) were obtained at 60μgL−1 level of sulfadiazine for intra- and inter-day analysis respectively. The method was successfully applied to determine sulfadiazine in honey, milk, human urine and environmental water samples.