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A chemiluminescence method for screening of fluoroquinolones in milk samples based on a multi-pumping flow system
- Vakh, Christina, Pochivalov, Aleksei, Koronkiewicz, Stanislawa, Kalinowski, Slawomir, Postnov, Viktor, Bulatov, Andrey
- Food chemistry 2019 v.270 pp. 10-16
- automation, chemiluminescence, cost effectiveness, detection limit, fluoroquinolones, magnetism, milk, nanoparticles, screening, sorbents
- An automated and miniaturized chemiluminescence method for screening of fluoroquinolones in milk samples was proposed. The method was based on magnetic dispersive micro-solid phase extraction of analytes followed by the chemiluminescence determination of the total fluoroquinolones content using a multi-pumping flow system. In the developed method, Zr-Fe-C magnetic nanoparticles were used as an efficient sorbent for separation of fluoroquinolones from sample matrix. The chemiluminescence intensity obtained for Ce4+-SO32−-Tb3+ chemiluminescence system was greatly increased in the presence of the analytes. Under the optimal conditions, the detector response for fluoroquinolones was linear in the concentration ranges from 5·10−9 to 1·10−6 mol L−1 with respect to fleroxacin. The limit of detection, calculated from a blank test based on 3σ, was 2·10−9 mol L−1 with respect to fleroxacin. The presented method demonstrated to be a good tool for available and cost-effective point-of-need screening fluoroquinolones in milk samples.