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A chemiluminescence method for screening of fluoroquinolones in milk samples based on a multi-pumping flow system

Vakh, Christina, Pochivalov, Aleksei, Koronkiewicz, Stanislawa, Kalinowski, Slawomir, Postnov, Viktor, Bulatov, Andrey
Food chemistry 2019 v.270 pp. 10-16
automation, chemiluminescence, cost effectiveness, detection limit, fluoroquinolones, magnetism, milk, nanoparticles, screening, sorbents
An automated and miniaturized chemiluminescence method for screening of fluoroquinolones in milk samples was proposed. The method was based on magnetic dispersive micro-solid phase extraction of analytes followed by the chemiluminescence determination of the total fluoroquinolones content using a multi-pumping flow system. In the developed method, Zr-Fe-C magnetic nanoparticles were used as an efficient sorbent for separation of fluoroquinolones from sample matrix. The chemiluminescence intensity obtained for Ce4+-SO32−-Tb3+ chemiluminescence system was greatly increased in the presence of the analytes. Under the optimal conditions, the detector response for fluoroquinolones was linear in the concentration ranges from 5·10−9 to 1·10−6 mol L−1 with respect to fleroxacin. The limit of detection, calculated from a blank test based on 3σ, was 2·10−9 mol L−1 with respect to fleroxacin. The presented method demonstrated to be a good tool for available and cost-effective point-of-need screening fluoroquinolones in milk samples.