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Synthesis of omega-3 ethyl esters from chia oil catalyzed by polyethylene glycol-modified lipases with improved stability

Author:
Castejón, Natalia, Moreno-Pérez, Sonia, Abreu Silveira, Erick, Fernández Lorente, Gloria, Guisán, José M., Señoráns, Francisco J.
Source:
Food chemistry 2019 v.271 pp. 433-439
ISSN:
0308-8146
Subject:
Salvia hispanica, Thermomyces lanuginosus, adsorption, biocatalysts, carboxylic ester hydrolases, catalytic activity, coatings, esters, ethanolysis, fatty acids, hydrophobicity, industrial applications, liquids, polyethylene, seed oils
Abstract:
Enzymatic synthesis of fatty acid ethyl esters (FAEE) from chia (Salvia hispanica L.) oil has been performed with different modified derivatives and compared with commercial immobilized lipases to produce omega-3 rich FAEE. Therefore, the main objective was to synthesize omega-3 esters from chia oil catalysed by polyethylene glycol-modified lipases using a biocatalyst with higher stability than commercial derivatives. Lipase from Thermomyces lanuginosus (TLL) was immobilized by hydrophobic adsorption on Sepabeads C-18 followed by a physicochemical coating of lipase surface with a dense layer of PEG. Ethanolysis reactions were carried out using pressurized liquid extracted chia seed oil and with different lipase derivatives to compare the omega-3 FAEE yield and ratio of reaction products, which were analysed by HPLC-ELSD. Furthermore, reutilization of lipase derivatives was studied to evaluate the stability after several reaction cycles to minimize decreasing of catalytic activity and develop a feasible enzymatic process for food industrial applications.
Agid:
6109479