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An in vitro and in silico study on the antioxidant and cell culture-based study on the chemoprotective activities of fish muscle protein hydrolysates obtained from European seabass and gilthead seabream

Author:
Altınelataman, Can, Koroleva, Olga, Fedorova, Tatyana, Torkova, Anna, Lisitskaya, Ksenia, Tsentalovich, Mikhail, Kononikhin, Aleksey, Popov, Igor, Vasina, Daria, Kovalyov, Leonid, Çelik, Ufuk
Source:
Food chemistry 2019 v.271 pp. 724-732
ISSN:
0308-8146
Subject:
Dicentrarchus labrax, Sparus aurata, antioxidant activity, antioxidants, cell culture, chemoprevention, chymotrypsin, colon, fish, human cell lines, hydrolysates, muscle protein, muscles, neoplasm cells, oxidative stress, peptides, protein hydrolysates, raw materials, subtilisin
Abstract:
European seabass (Dicentrarchus labrax, Linnaeus, 1758) (L) and gilthead seabream (Sparus aurata, Linnaeus, 1758) (C) muscles were hydrolysated by Alcalase (Lₐₗc, Cₐₗc) and Chymotrypsin (Lcₕ, Ccₕ) then hydrolysates were examined and their peptide profiles obtained. A total of 765, 794, 132 and 232 peptides were identified in Cₐₗc, Lₐₗc, Ccₕ and Lcₕ, respectively. Although, Lcₕ and Ccₕ were expected to have more antioxidant capacity because of their peptide profiles, Alcalase hydrolysates observed in vitro, were slightly higher (TEAC assay for Cₐₗc: 848.11 ± 60.78 μmol TE/g protein). Maximum inhibition of oxidative stress was determined for Lₐₗc (12.8% ± 4.5%) in MDCK1 cell lines. Highest proliferative capacity observed for Cₐₗc (147.0% ± 3.1%) at MTT assay in MDCK1 cell culture. Lcₕ showed the highest chemopreventive effect with a 40–60% decrease for human colon adenocarcinoma cell line HT-29. This research points out the importance of aquatic sources as raw materials for peptide researches.
Agid:
6109489