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Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo
- Qi, Yi, Zhang, Bingquan, Wang, Junting, Yao, Mengna
- Gene 2018 v.676 pp. 79-85
- animal experimentation, apoptosis, cell growth, cell lines, cell proliferation, circular RNA, gain-of-function mutation, gene expression regulation, loss-of-function mutation, lung neoplasms, lymph nodes, metastasis, patients, prognosis, quantitative polymerase chain reaction, regression analysis, reverse transcriptase polymerase chain reaction, therapeutics, tissues
- Emerging evidence documented the key functions of circular RNAs (circRNAs) in various malignancies. Nevertheless, the research relevant to the clinical value and functions of circRNAs in non-small cell lung cancer (NSCLC) is still very limited. In this study, we performed qRT-PCR to assess the levels of hsa_circ_0007534 in NSCLC tissues and cell lines and evaluated its clinical significance using Fisher's exact test, Kaplan-Meier analysis and Cox regression analysis. Additionally, loss-of-function and gain-of-function experiments were performed to detect whether hsa_circ_0007534 could affect cell proliferation, apoptosis, metastatic properties and epithelial-mesenchymal transition (EMT) in A549 and H1299 cells. Further xenograft study was carried out to validate the in vitro data. The results indicated that hsa_circ_0007534 was up-regulated in both NSCLC tissue samples and cell lines and this up-regulation is linked to lymph node invasion and advanced TNM stage. Hsa_circ_0007534 could also function as an unfavorable prognostic indicator for the patients with NSCLC. For the part of functional assays, down-regulation of hsa_circ_0007534 suppressed cell growth, migratory and invasive capacities; facilitating cell apoptosis in A549 and H1299 cells. Conversely, up-regulation of hsa_circ_0007534 caused the opposite biological behaviors. What's more, animal experiments validated the oncogenic role of hsa_circ_0007534. Ultimately, the present study indicates that hsa_circ_0007534 might be a potential NSCLC-associated prognostic/therapeutic target.