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DNA methylation status of CRABP2 promoter down-regulates its expression

Gui-Min Zhang, Cheng-Chuang Song, Li-Juan Li, Hua He, Shu-Yue Shi, Chu-Zhao Lei, Li Zheng, Shu-Jun Peng, Yi-Ran Tian, Rui-Hua Dang, Xian-Yong Lan, Xing-Lei Qi, Hong Chen, Yong-Zhen Huang
Gene 2018 v.676 pp. 243-248
DNA methylation, absorption, adipocytes, beef quality, binding proteins, bisulfites, cattle, cattle breeds, cell differentiation, epigenetics, fibroblasts, gene expression, gene expression regulation, genes, genomic islands, homeostasis, hybrids, messenger RNA, metabolism, methyltransferases, muscles, neoplasms, phenotype, purebreds, quantitative polymerase chain reaction, retinoic acid, sequence analysis, therapeutics, transcription (genetics)
As an important epigenetic modification DNA methylation is catalyzed by DNA methylation transferases (DNMTs) and occurs mainly in CpG islands. DNA methylation plays an important role in regulates gene expression, cell differentiation, genetic imprinting and tumor therapy. Retinoic acid-binding proteins (RAC) is vital for the absorption, transport, metabolism and maintenance of homeostasis of retinoic acid, which in turn regulates the differentiation and proliferation of cells by regulating the transcription of many target genes, therefore, these proteins influence differentiation and proliferation of adipocytes and muscle fibroblasts. Thus, cellular retinoic acid binding protein 2 (CRABP2) may be a candidate gene which affects beef quality, yield and fat deposition. The aim of this study was to evaluate the expression and the methylation pattern on the differentially methylated region (DMR) of the promoter of CRABP2. The DNA methylation pattern was tested by bisulfite sequencing polymerase chain reaction (BSP), the quantitative real-time PCR (qPCR) was used to analysis the expression of CRABP2 gene. The results showed that the DNA methylation level was higher in purebred cattle breed than that in hybrid cattle breeds which was negative correlation with the expression of the gen. These results indicate that the methylation status of the CRABP2 DMR can regulate mRNA expression. What's more, there are different methylation and expression patterns in different breeds and tissues which may influence the phenotype, and the results may be a useful parameter to investigate the function of CRABP2 in muscle and fat developmental in Chinese cattle.