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Comparison of Superdex Peptide HR 10/30 Column and TSK Gel G2000 SWXL Column for Molecular Weight Distribution Analysis of Protein Hydrolysates

Author:
Su, Guowan, Ren, Jiaoyan, Zhao, Mouming, Sun, Da-Wen
Source:
Food and bioprocess technology 2013 v.6 no.12 pp. 3620-3626
ISSN:
1935-5130
Subject:
chromatography, gels, molecular weight, peanut protein, peptides, pork, protein hydrolysates, ultrafiltration
Abstract:
Superdex Peptide HR 10/30 column and TSK gel G2000 SWXL column were compared for molecular weight (MW) distribution analysis of pure proteins/peptides standards, pork/peanut protein hydrolysates, and their peptide fractions. Results revealed that good chromatographic resolution was observed for pure proteins/peptides standards in the MW range of 189 Da to 12,384 Da on Superdex Peptide HR 10/30 column, while TSK gel G2000 SWXL column is more suitable for pure standard in the region of 6,500 Da to 75,000 Da. The test of MW distribution of peptide fractions by ultrafiltration or Sephadex G-25 chromatography indicated that Superdex Peptide HR 10/30 column had better chromatographic resolution for peptides below 3,000 Da than TSK gel G2000 SWXL column. ©Springer Science+Business Media, LLC 2012.
Agid:
611064