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Transcriptional response to petiole heat girdling in cassava

Author:
Zhang, Yang, Ding, Zehong, Ma, Fangfang, Chauhan, Raj Deepika, Allen, Doug K., Brutnell, Thomas P., Wang, Wenquan, Peng, Ming, Li, Pinghua
Source:
Scientific reports 2015 v.5 no.8414
ISSN:
2045-2322
Subject:
Manihot esculenta, biochemical pathways, biosynthesis, cassava, chlorophyll, crop yield, electron transfer, flavonoids, gene expression, genes, girdling, heat, homeostasis, mitochondria, nitrogen metabolism, petioles, photoperiod, photosynthesis, starch, sugars, transcription (genetics), transcriptome
Abstract:
The heat-girdling technique, which is known to inhibit photoassimilate translocation, was performed on the petiole of cassava leaves at the end of the light cycle to inhibit starch remobilization during the night. The inhibition of starch remobilization caused significant starch accumulation at the beginning of the light cycle, which inhibited photosynthesis and affected intracellular sugar levels. Changes in photosynthesis and sugar levels may influence the transcriptome, and the genes that respond to these changes may be important for understanding homeostasis regulation between source supply and sink demand. Based on RNA-Seq comparison of leaves with or without heat girdling treatment, we observed significantly decreased expression of genes related to photosynthesis, N-metabolism and chlorophyll biosynthesis. However, expression of genes TCA and mitochondria electron transport, as well as flavonoid biosynthetic pathways, were induced significantly. Changes in primary and secondary metabolism, as well as sugar and light signaling, may increase our understanding of the relationship between source and sink and provide target genes for future bio-engineering to further improve cassava yield.
Agid:
61126
Handle:
10113/61126