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Microbial diversity associated to the intestinal tract of soil invertebrates

da Silva Correia, Dayana, Passos, Samuel Ribeiro, Proença, Diogo Neves, Morais, Paula Vasconcelos, Xavier, Gustavo Ribeiro, Correia, Maria Elizabeth Fernandes
Applied soil ecology 2018 v.131 pp. 38-46
Actinobacteria, Bacteroidetes, Buttiauxella, Enterobacter, Kluyvera, Pantoea, Pycnoscelus surinamensis, Serratia, Trigoniulus lumbricinus, bacteria, bacterial communities, carbon, denaturing gradient gel electrophoresis, detritivores, developmental stages, diet, intestinal microorganisms, intestines, molecular cloning, nitrogen, organic matter, phylogeny, polymerase chain reaction, prokaryotic cells, ribosomal DNA, sequence analysis, soil ecosystems, soil invertebrates
Interactions between saprophagous invertebrates and microbes are essential for the maintenance and functioning of soil ecosystems, as they directly affect the degradation of organic matter and the nutrient cycle. The intestinal tract of invertebrates is inhabited by a diversity of microbes, and it is closely associated with the food ingested. The aim of this work was to evaluate the profile of prokaryotes associated with the intestinal tract of three invertebrate species. The species of invertebrates Trigoniulus corallinus was collected and incubated in the experiment, after 5 days of incubation we observed the uninduced colonization of two invertebrate species Cubaris murina and Pycnoscelus surinamensis. Therefore, the three species were evaluated in the same way, after 60 days of incubation. The diet supplied comprised different vegetal residues, with distinct carbon/nitrogen compositions. Six treatments were evaluated. After 60 days, five individuals of each species were randomly selected, by removing the posterior third of the intestinal tract. These specimens were next subjected to DNA extraction. The PCR/DGGE analysis was carried out using the 16S rDNA, for the domain Bacteria and the phylum Actinobacteria. DGGE bands were cloned and sequenced using the Bacterial domain. In multivariate analyzes, individuals of the same species after 60 days of incubation, were strongly grouped. These results may be in accordance with the environmental criteria of the host itself, stage of development, phylogeny and diet. Thus, the investigation of the intestinal microbiota, provides relationships between invertebrates and their intestinal bacterial communities. In view of this information, we used the technique of sequencing cloned DGGE bands to quantify the diversity of microorganisms present in the intestinal tract of the studied invertebrates. The phylum Firmicutes, Bacteroidetes, and Proteobacteria were identified by sequencing the cloned bands; Proteobacteria presented the highest number of genera, comprising Enterobacter, Buttiauxella, Serratia, Kluyvera, and Pantoea.