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Towards better understanding of industrial cell factories: novel approaches for 13C metabolic flux analysis in complex nutrient environments
- Schwechheimer, Susanne Katharina, Becker, Judith, Wittmann, Christoph
- Current opinion in biotechnology 2018 v.54 pp. 128-137
- carbon, hosts, metabolic engineering, metabolic flux analysis, stable isotopes
- The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medium compounds. The use of parallel 13C tracer experiments as well as analytical multi-readout of labeling data together with model-based simulation offer a great starting point to tackle such complex systems. Prominent examples will highlight the recent progress made.