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Cryosurvival after exposure of IVF-derived Nellore embryos to different cryoprotectants and exposure times during vitrification
- Souza, Juliano F., Lienou, Landry L., Rodrigues, Ana P.R., Alexandrino, E., Cavalcante, Tania V., Santos, Regiane R., Figueiredo, Jose R., Dias, Francisca Elda F.
- Cryobiology 2018 v.84 pp. 95-97
- Nellore, blastocyst, cryoprotectants, dimethyl sulfoxide, ethylene glycol, exposure duration, hatching, propylene glycol, vitrification, zebu
- We evaluated the effects of the vitrification solution (i.e., ethylene glycol (EG) + dimethyl sulfoxide (DMSO) with or without propylene glycol (PG)) and of exposure time on the re-expansion and hatching rates of vitrified Bos indicus embryos. In vitro produced embryos (n = 1050) were divided into seven groups: control group (non-vitrified embryos) and six vitrification groups with different cryoprotectant concentrations and exposure times. After vitrification, embryos were cultured for determination of re-expansion and hatching rates. Vitrification with 25% DMSO +25% EG (exposure for 1 min and 20 s) resulted in the highest re-expansion (65.2%) and hatching (68.2%) rates. The lowest re-expansion and hatching rates were observed in vitrification with 12.5% DMSO + 25% EG + 12.5% PG with both tested exposure times (i.e., 3 min + 1 min and 1 min + 20 s). A combination of DMSO + EG is efficient to preserve blastocysts, especially following a short exposure time.