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Cryosurvival after exposure of IVF-derived Nellore embryos to different cryoprotectants and exposure times during vitrification

Souza, Juliano F., Lienou, Landry L., Rodrigues, Ana P.R., Alexandrino, E., Cavalcante, Tania V., Santos, Regiane R., Figueiredo, Jose R., Dias, Francisca Elda F.
Cryobiology 2018 v.84 pp. 95-97
Nellore, blastocyst, cryoprotectants, dimethyl sulfoxide, ethylene glycol, exposure duration, hatching, propylene glycol, vitrification, zebu
We evaluated the effects of the vitrification solution (i.e., ethylene glycol (EG) + dimethyl sulfoxide (DMSO) with or without propylene glycol (PG)) and of exposure time on the re-expansion and hatching rates of vitrified Bos indicus embryos. In vitro produced embryos (n = 1050) were divided into seven groups: control group (non-vitrified embryos) and six vitrification groups with different cryoprotectant concentrations and exposure times. After vitrification, embryos were cultured for determination of re-expansion and hatching rates. Vitrification with 25% DMSO +25% EG (exposure for 1 min and 20 s) resulted in the highest re-expansion (65.2%) and hatching (68.2%) rates. The lowest re-expansion and hatching rates were observed in vitrification with 12.5% DMSO + 25% EG + 12.5% PG with both tested exposure times (i.e., 3 min + 1 min and 1 min + 20 s). A combination of DMSO + EG is efficient to preserve blastocysts, especially following a short exposure time.