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Extraction of anthocyanins from haskap berry pulp using supercritical carbon dioxide: Influence of co-solvent composition and pretreatment
- Jiao, Guangling, Kermanshahi pour, Azadeh
- Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.98 pp. 237-244
- anthocyanins, antioxidant activity, carbon dioxide, ethanol, experimental design, freeze drying, pulp, temperature
- Extracts rich in anthocyanin compounds were obtained from haskap berry pulp paste using supercritical carbon dioxide (scCO2) and water as co-solvent. The extraction conditions including pressure, temperature, and amount of water were further optimized by Box-Behnken design. The highest total anthocyanins (TA) yield of 52.7% was achieved at 45 MPa, 65 °C, 5.4 g water to 3.2 g berry pulp paste, 15 min static and 20 min dynamic time. Different combinations of water and ethanol as co-solvent did not significantly affect the TA yield. Furthermore, similar anthocyanin extraction yields were obtained in the case of the pulp paste and rehydrated freeze-dried berry pulp powder, which indicates that freeze drying pretreatment is not required prior to scCO2 extraction. Compared with conventional extraction, the use of scCO2 and water as co-solvent offered higher anthocyanin extraction efficiency (52.7% versus 38.3%) with improved antioxidant activity (89.8% versus 72.2%).