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Achromobacter denitrificans SP1 produces pharmaceutically active 25C prodigiosin upon utilizing hazardous di(2-ethylhexyl)phthalate
- S. Pradeep, M.K. Sarath Josh, S. Balachandran, R. Sudha Devi, R. Sadasivam, P.E. Thirugnanam, Mukesh Doble, Robin C. Anderson, Sailas Benjamin
- Bioresource technology 2014 v.171 pp. 482-486
- Achromobacter, binding capacity, carbon, cell culture, enzyme activation, molecular models, pH, phthalates, pigments, plastics, prostaglandin synthase, protein kinases, sewage sludge
- This first report describes the purification and identification of an orange-red pigment produced by Achromobacter denitrificans strain SP1 (isolated from sewage sludge heavily contaminated with plastics) during its growth in a simple basal salt medium supplemented with the hazardous di(2-ethylhexyl)phthalate (DEHP) blended in PVC blood bag (in situ) or free DEHP (ex situ) as carbon source. The cell-bound pigment was elucidated, characterized at molecular level, and described as an unusual 25C prodigiosin analog for the first time. At laboratory conditions (in flasks), the dry cell mass was 75.2mg/g blood bag, which upon extraction yielded 7.1mg prodigiosin; at this stage the pH of the medium was dropped from 7.2 to 3.5. Considering its pharmaceutical importance, taking 10 known prodigiosins as controls, this 25C prodigiosin was subjected to molecular docking studies, showed comparable and promising binding efficiencies with the crucial molecular human targets like cycloxygenase-2, ZAP-70 kinase and Jak-3 kinase.