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Fipronil causes toxicity in mouse preimplantation embryos

Šefčíková, Zuzana, Babeľová, Janka, Čikoš, Štefan, Kovaříková, Veronika, Burkuš, Ján, Špirková, Alexandra, Koppel, Juraj, Fabian, Dušan
Toxicology 2018 v.410 pp. 214-221
blastocyst, blastomeres, cell death, embryogenesis, embryotoxicity, females, fipronil, fluorescence, in vitro studies, in vivo studies, ingredients, mice, oral administration, staining
In this study the possible toxicity of phenylpyrazole fipronil, the related commercial product FIPRON spot-on as well as FIPRON spot-on secondary ingredients on the developmental capacities and quality of mouse preimplantation embryos was evaluated.During in vitro tests, isolated two-cell stage embryos were cultured in media with addition of the listed chemicals until blastocyst formation. Stereomicroscopic evaluation of in vitro produced embryos showed that fipronil at 1 μM and higher concentration negatively affected embryonic development. Fluorescence staining revealed that the obtained blastocysts displayed lower numbers of blastomeres at 10 μM concentrations and elevated incidence of cell death from 1 μM concentration. The presence of FIPRON spot-on at a concentration equivalent to 10 μM of fipronil caused massive degeneration of all embryos. Secondary ingredients (butylhydroxyanisolum, butylhydroxytoluenum) at corresponding concentrations negatively impacted the development and quality of preimplantation embryos as well.During in vivo tests (daily oral administration of fipronil during the preimplantation period) in embryos collected from treated mouse females, significantly elevated incidence of cell death was observed even at the acute reference dose.Fipronil impaired the development and quality of mouse preimplantation embryos in both in vitro and in vivo tests. Embryotoxicity of the commercial product FIPRON spot-on was potentiated by the presence of secondary ingredients.