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Lignification plays an important role on resistance to root-knot nematode (Meloidogyne incognita) based on contrastive analysis in peach

Author:
Wang, Qi, Cao, Ke, Li, Haiyan, Zhu, Gengrui, Fang, Weichao, Chen, Changwen, Wang, Xinwei, Wang, Lirong
Source:
Scientia horticulturae 2018 v.238 pp. 1-6
ISSN:
0304-4238
Subject:
Meloidogyne incognita, biosynthesis, cultivars, gene expression regulation, gene ontology, genes, genotype, histology, lignification, lignin, peaches, root-knot nematodes, roots, transcription (genetics), transcriptomics
Abstract:
Root-knot nematode (RKN) is a serious global pest in peach cultivation; it establishes and maintains a permanent feeding site within plant roots. To elucidate the host response to RKN infection in peach, we conducted a comparative histological and transcriptome analysis during RKN invasion in two genotypes of peach–‘Honggengansutao’ (resistant to Meloidogyne incognita) and ‘Bailey’ (susceptible genotype). Roots of the two genotypes infested with nematodes for 0, 12, 36 and 84 h were taken as samples. Histological analysis showed that cells concentrated with RKN were lignified. Gene ontology analysis revealed a higher percentage of differential expressed genes enriched for “catalytic” and “metabolic process”. Therefore, genes with catalytic functions in lignin biosynthesis were focused on. Filtering of genes with no obvious differences compared to control revealed the resistant cultivar responded to RKN infection earlier than the susceptible one; two genes involved in upstream of lignin biosynthesis pathway were up-regulated and an additional GT-1 cis-element which can activate transcription in vivo was found in each promoter of these two genes only in the resistant cultivar. These results form a basis for understanding the mechanism of RKN resistance in peach and other plants.
Agid:
6119514