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A unified approach for high-throughput quantitative analysis of the residues of multi-class veterinary drugs and pesticides in bovine milk using LC-MS/MS and GC–MS/MS

Jadhav, Manjusha R., Pudale, Anjali, Raut, Prakash, Utture, Sagar, Ahammed Shabeer, T.P., Banerjee, Kaushik
Food chemistry 2019 v.272 pp. 292-305
acetonitrile, cleaning, gas chromatography, guidelines, magnesium sulfate, milk, multiresidue analysis, organochlorine pesticides, penicillins, quantitative analysis, quinolones, sodium chloride, tandem mass spectrometry, tetracyclines, ultrafast liquid chromatography, veterinary drugs
This paper reports a unified sample preparation approach for high-throughput multi-residue analysis of veterinary drugs and pesticides in a single sub-sample of bovine milk. The sample (5 g) was deproteinized with acetonitrile before an aliquot (I) was withdrawn, and the remainder was phase-separated using MgSO4 and NaCl. The acetonitrile layer (II) was recovered and the extracts combined, cleaned, and solvent-exchanged before the concentrations of veterinary drugs and pesticides were measured by ultra-fast liquid chromatography tandem mass spectrometry (UFLC-MS/MS). As a unique approach, extract II was analyzed simultaneously using gas chromatography tandem mass spectrometry (GC–MS/MS). Method performance for 78 drugs and 238 pesticides complied with CD 2002/657/EC and SANTE/11813/2017 guidelines, respectively, with significant savings in time and cost. Thus, it would be ideal for regulatory analysis of analytes ranging from non-polar organochlorine pesticides to polar drugs, including penicillins, quinolones, and tetracyclines.