U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Aspergillus flavus resident in Kenya: High genetic diversity in an ancient population primarily shaped by clonal reproduction and mutation-driven evolution

Author:
Md-Sajedul Islam, Kenneth A. Callicott, Charity Mutegi, Ranajit Bandyopadhyay, Peter J. Cotty
Source:
Fungal ecology 2018 v.35 pp. 20-33
ISSN:
1754-5048
Subject:
Aspergillus flavus, alleles, asexual reproduction, evolution, genetic analysis, genetic distance, genetic variation, haplotypes, in vitro studies, linkage disequilibrium, loci, meiosis, microsatellite repeats, soil, soil sampling, Kenya
Abstract:
Aspergillus flavus has long been considered to be an asexual species. Although a sexual stage was recently reported for this species from in vitro studies, the amount of recombination ongoing in natural populations and the genetic distance across which meiosis occurs is largely unknown. In the current study, genetic diversity, reproduction and evolution of natural A. flavus populations endemic to Kenya were examined. A total of 2744 isolates recovered from 629 maize-field soils across southern Kenya in two consecutive seasons were characterized at 17 SSR loci, revealing high genetic diversity (9-72 alleles/locus and 2140 haplotypes). Clonal reproduction and persistence of clonal lineages predominated, with many identical haplotypes occurring in multiple soil samples and both seasons. Genetic analyses predicted three distinct lineages with linkage disequilibrium and evolutionary relationships among haplotypes within each lineage suggesting mutation-driven evolution followed by clonal reproduction. Low genetic differentiation among adjacent communities reflected frequent short distance dispersal.
Agid:
6123112
Handle:
10113/6123112