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Gamma-conglutin peptides from Andean lupin legume (Lupinus mutabilis Sweet) enhanced glucose uptake and reduced gluconeogenesis in vitro

Muñoz, Erika B., Luna-Vital, Diego A., Fornasini, Marco, Baldeón, Manuel E., Gonzalez de Mejia, Elvira
Journal of functional foods 2018 v.45 pp. 339-347
Lupinus mutabilis, adipocytes, dipeptidyl-peptidase IV, enzyme activity, gluconeogenesis, glucose, glucose transporters, hydrolysates, hydrolysis, insulin receptors, legumes, mechanism of action, models, peptides, Andes region
The present study aimed to analyze the mechanism of action of a hydrolyzed gamma-conglutin fraction (Cgh) extracted from Andean L. mutabilis legume on glucose metabolism. At 5 mg/mL, Cgh from L. mutabilis inhibited dipeptidyl peptidase IV activity (DPP-IV) (100%), and increased (p < 0.05) 6.5-fold the glucose uptake in a dual-layered enterocyte/adipocyte culture system compared to untreated cells, through an enhancement of glucose transporter type-4 translocation. In this model, insulin-induced glucose uptake was potentiated by addition of L. mutabilis hydrolysates. Also, Cgh L. mutabilis hydrolysates decreased (p < 0.05) by 50% gluconeogenesis in a dual-layered enterocyte/hepatocyte system and reduced phosphoenolpyruvate carboxykinase expression. These data showed that L. mutabilis hydrolysates can affect glucose metabolism inhibiting DPP-IV enzymatic activity, improving insulin receptor sensitivity and inhibiting hepatic gluconeogenesis.