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Pilot‐scale Protamex™‐catalysed production of round scad protein hydrolysates:effects of agitation alone and combined with aeration

Yao, Yujing, Yang, Juan, Sun‐Waterhouse, Dongxiao, Cui, Chun, Wang, Wei, Dong, Keming
International journal of food science & technology 2018 v.53 no.10 pp. 2308-2315
aeration, agitation, anaerobes, bacteria, cadaverine, hydrolysis, nitrogen, plate count, protein degradation, protein hydrolysates, proteolysis, raw materials
A pilot‐scale production of round scad protein hydrolysates via Protamex™‐catalysed proteolysis was firstly established. Process optimisation then focused on the use of mechanical agitation (MA) alone or combined with aeration (MAA). Comparative examinations revealed that proteolysis involving either MA or MAA was feasible with a hydrolysis degree up to 55%, and individual and total biogenic amine (BA) contents much lower than the commonly recognised limits (including the dominant BA, cadaverine). MAA is more advantageous in suppressing bacterial counts and activities associated with protein decomposition, enabling reduced volatile basic nitrogen and BAs. Thus, additional aeration to mechanical agitation is recommended for industrial proteolysis of raw materials unavoidably containing micro‐organisms (especially anaerobic bacteria) in common handling practice.