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Detection of Hammondia heydorni DNA in feces collected in and around an Ohio Wildlife Conservation Center

Author:
Sinnott, D., Moreno Torres, K., Wolfe, B., Garabed, R., Marsh, A.E.
Source:
Veterinary Parasitology: Regional Studies and Reports 2016 v.6 pp. 31-34
ISSN:
2405-9390
Subject:
Canidae, DNA, Hammondia heydorni, feces, hosts, melting curve analysis, oocysts, parasites, polymerase chain reaction, wildlife, Ohio
Abstract:
This study aimed to identify DNA attributed to Hammondia heydorni oocysts in the feces of wild canids in and around an Ohio wildlife conservation center. Two hundred and eighty-five wild canid fecal samples were analyzed using PCR with melting curve analysis to detect coccidian DNA. Coccidia-positive samples were further subjected to H. heydorni-specific and N. caninum-specific PCR assays. Samples positive by the H. heydorni-specific assay were additionally analyzed with a PCR assay to distinguish H. heydorni from Hammondia triffittae. Coccidian DNA was detected in 51 of the 285 (17.9%) wildlife samples. H. heydorni DNA was detected in three of the coccidia-positive wildlife samples (1.1%) and N. caninum was detected in none. Determining the presence of H. heydorni in wild canids will contribute to a greater understanding of the role these hosts play in the ecology of this parasite.
Agid:
6127787