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Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia
- Zhang, Dan, Mao, Haiyan, Lou, Xiuyu, Pan, Junhang, Yan, Hao, Tang, Hongfeng, Shu, Yan, Zhao, Yun, Cheng, Xiaoli, Tao, Hong, Zhang, Yanjun, Ma, Xuejun
- Archives of virology 2018 v.163 no.10 pp. 2855-2860
- clinical examination, instrumentation, monitoring, pneumonia, quantitative analysis, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, viruses, China
- We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a previously reported two-tube mRT-PCR assay using 363 clinical sputum specimens. The mqRT-PCR assay performed comparably with the two-tube assay for most viruses, offering the advantages of quantitative analysis, easier performance, lower susceptibility to contamination, and shorter turnaround time in laboratories equipped with conventional real-time PCR instrumentation, and it could therefore be a valuable tool for routine surveillance of respiratory virus infections in China.