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Detection of bovine herpesvirus 1 in cumulus-oocyte complexes of cows
- Queiroz-Castro, Vanessa L.D., da Costa, Eduardo P., Alves, Saullo Vinicius P., Júnior, Abelardo Silva, Machado-Neves, Mariana, Guimarães, José D.
- Research in veterinary science 2018 v.120 pp. 54-56
- Bovine herpesvirus 1, animal ovaries, blood sampling, confocal laser scanning microscopy, cows, cytoplasm, fluorescent antibody technique, infectious bovine rhinotracheitis, plant diseases and disorders, plant viruses, serodiagnosis, seroprevalence, signs and symptoms (animals and humans)
- Bovine herpesvirus 1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis (IBR) and is also associated with reproductive failure. This study investigated the presence of BoHV-1 in cumulus-oocyte complexes (COCs) of naturally-infected cows without clinical signs of IBR. The presence of BoHV-1 in COCs was evaluated by immunofluorescence using confocal laser scanning microscopy. Blood samples and ovaries from 82 cows that had not been vaccinated against BoHV-1 were collected for serological analysis. COCs were divided into two pools: COCs derivate from seropositive cows and from seronegative cows. Then, the samples were processed for confocal microscopy analysis. The results indicated that 61% (50/82) of cows were seropositive for BoHV-1. A total of 719 COCs were obtained from the cows and processed. None of 276 COCs from the 32 seronegative cows presented BoHV-1. However, BoHV-1 was present in the cytoplasm of cumulus cells from 158 out of 443 COCs aspirated from the seropositive cows. The detection of BoHV-1 in the COCs of seropositive cows suggests that the COCs of naturally-infected, asymptomatic cows may be infected with BoHV-1.