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Anti-glioma properties of DVL, a lectin purified from Dioclea violacea

Nascimento, Ana Paula M., Knaut, Jhônatas L., Rieger, Débora K., Wolin, Ingrid A.V., Heinrich, Isabella A., Mann, Josiane, Juarez, Andrea V., Sosa, Liliana del V., De Paul, Ana Lucia, Moreira, Cleane G., Silva, Ivanice B., Nobre, Clareane S., Osterne, Vinicius J.S., Nascimento, Kyria S., Cavada, Benildo S., Leal, Rodrigo B.
International journal of biological macromolecules 2018 v.120 pp. 566-577
Canavalia, Dioclea, antineoplastic activity, apoptosis, autophagy, caspase-3, cell lines, cell movement, cell viability, concanavalin A, membrane potential, mitochondrial membrane, rats, seeds
Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well-studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity.