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A myeloid differentiation factor 88 gene from yellow catfish Pelteobagrus fulvidraco and its molecular characterization in response to polyriboinosinic polyribocytidylic acid and lipopolysaccharide challenge

Zhao, Xiao-Ming, Chu, Xiao-Hua, Liu, Yu, Liu, Qiu-Ning, Jiang, Sen-Hao, Zhang, Dai-Zhen, Tang, Bo-Ping, Zhou, Chun-Lin, Dai, Li-Shang
International journal of biological macromolecules 2018 v.120 pp. 1080-1086
Danio rerio, Homo sapiens, Ictalurus punctatus, Tachysurus fulvidraco, Toll-like receptors, amino acid sequences, blood, complementary DNA, freshwater fish, genes, innate immunity, kidneys, lipopolysaccharides, liver, muscles, open reading frames, polyinosinic-polycytidylic acid, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, signal transduction, spleen, tissues
Myeloid differentiation factor 88 (MyD88) is an adaptor protein of Toll-like receptor (TLR) signalling pathways that activates the innate immune system. Herein, MyD88 was identified in the economically important freshwater fish Pelteobagrus fulvidraco. The complete 2156 bp PfMyD88 cDNA includes a 147 bp 5′-untranslated region (UTR), a 1133 bp 3′-UTR, and an open reading frame (ORF) of 876 bp encoding a 291 residue protein containing Death and Toll/interleukin-1 receptor (TIR) domains. The deduced protein sequence shares 88.8%, 73.8% and 59.3% identity with orthologs in Ictalurus punctatus, Danio rerio and Homo sapiens, respectively. qRT-PCR revealed expression in all tested tissues, highest in trunk kidney, followed by spleen, and lowest in muscle. After challenge with lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid (Poly I:C), PfMyD88 expression was up-regulated in blood, liver, head kidney and spleen. Thus, PfMyD88 acts in innate immunity in P. fulvidraco.