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Studies of antibody-antigen interactions by capillary electrophoresis: A review

Author:
Moser, Annette C., Trenhaile, Sidney, Frankenberg, Kati
Source:
Methods 2018 v.146 pp. 66-75
ISSN:
1046-2023
Subject:
antibodies, antigen-antibody reactions, antigens, capillary electrophoresis, immunoassays
Abstract:
Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.
Agid:
6133159