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Antioxidant peptides derived from the hydrolyzate of purple sea urchin (Strongylocentrotus nudus) gonad alleviate oxidative stress in Caenorhabditis elegans

Author:
Zhao, Sixu, Cheng, Qiong, Peng, Qiong, Yu, Xuesong, Yin, Xiquan, Liang, Ming, Ma, Chung Wah, Huang, Zebo, Jia, Weizhang
Source:
Journal of functional foods 2018 v.48 pp. 594-604
ISSN:
1756-4646
Subject:
Caenorhabditis elegans, RNA interference, Strongylocentrotus nudus, Strongylocentrotus purpuratus, antioxidant activity, gel chromatography, genes, heat stress, high performance liquid chromatography, hydrolysates, oxidative stress, papain, peptides, reactive oxygen species, signal transduction, superoxide dismutase, tandem mass spectrometry, ultrafiltration
Abstract:
The papain hydrolyzate prepared from purple sea urchin (Strongylocentrotus nudus) gonad exerts significant antioxidant activity. Through ultrafiltration, gel filtration and high performance liquid chromatography, the active fractions from the hydrolyzate were screened by alleviating paraquat-induced oxidative stress in Caenorhabditis elegans, and the peptide components were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two antioxidant peptides SnP7 (AAVPSGASTGIYEALELR, 1805.03 Da) and SnP10 (NPLLEAFGNAK, 1173.34 Da) exhibit significant antioxidant capacity, which reduce reactive oxygen species (ROS) level and the expression of superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2) in oxidation-damaged nematodes. Further studies indicated that SnP7 and SnP10 induce DAF-16 nuclear translocation and the expression of stress-related genes such as sod-3. RNA interference (RNAi) suggested that peptide-induced enhancement of resistance is dependent on DAF-16. Taken together, S. nudus antioxidant peptides might act through activate DAF-16 signaling pathway, and induce the expression of DAF-16 target genes that enhance antioxidant potential of organisms.
Agid:
6133419