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Acetone/water oxidation of corn stover for the production of bioethanol and prebiotic oligosaccharides
- Katsimpouras, Constantinos, Dedes, Grigorios, Bistis, Perrakis, Kekos, Dimitrios, Kalogiannis, Konstantinos G., Topakas, Evangelos
- Bioresource technology 2018 v.270 pp. 208-215
- acetone, bioethanol, biomass, corn stover, distillation, enzymatic treatment, ethanol, ethanol production, fermentation, glucose, hemicellulose, liquefaction, mass transfer, mixing, oxidation, prebiotics, saccharification, slurries, viscosity, xylanases, xylooligosaccharides
- Ethanol production at high-gravity promise to achieve concentrations over the threshold for an economical distillation process and concurrently reduce water consumption. However, a persisting limitation is the poor mass transfer conditions resulting in low ethanol yields and concentrations. Hereby, the combination of an acetone/water oxidation pretreatment process (AWO) with a liquefaction/saccharification step, using a free-fall mixer, before simultaneous saccharification and fermentation (SSF) can realize ethanol concentrations of up to ca. 74 g/L at a solids content of 20 wt%. The free-fall mixer achieved a biomass slurry viscosity reduction by 87% after only 2 h of enzymatic saccharification, indicating the efficiency of the mixing system. Furthermore, the direct enzymatic treatment of AWO pretreated corn stover (CS) by a GH11 recombinant xylanase, led to the production of xylooligosaccharides (XOS) with prebiotic potential and the removal of insoluble fibers of hemicellulose improved the glucose release of AWOCS by 22%.