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Exposure to sublethal concentrations of tributyltin reduced survival, growth, and 20-hydroxyecdysone levels in a marine mysid

Kim, Bo-Mi, Saravanan, Manoharan, Lee, Do-Hee, Kang, Jung-Hoon, Kim, Moonkoo, Jung, Jee-Hyun, Rhee, Jae-Sung
Marine environmental research 2018 v.140 pp. 96-103
Malacostraca, acute toxicity, adults, antifouling agents, aquatic organisms, bioaccumulation, boats, ecdysterone, females, juveniles, mechanism of action, molting, mortality, neonates, paints, sublethal effects, toxicity testing, tributyltin
Tributyltin (TBT) is as an antifouling organotin compound used in boat paints. Although organotin-based antifouling agents have been banned on a global scale, the mode of action of TBT has been studied in numerous aquatic species because of its toxicity, persistence, bioaccumulation potential, and endocrine-disrupting characteristics. In this study, we conducted 96-h acute toxicity tests wherein we exposed juvenile and adult marine mysids to waterborne TBT. Over 4 weeks of exposure, mortality was dose-dependently increased in juveniles and adult mysids. To test sublethal effects of TBT on juvenile development, newborn juvenile mysids were exposed to 1, 5, or 10 ng L−1 TBT for 4 weeks. Subsequently, we measured morphological growth parameters and quantified the hormone ecdysterone (20-hydroxyecdysone: 20E), which controls molting in mysids. The lengths of the whole body, antennal scale, exopod, endopod, and telson were significantly smaller in the 5 and/or 10 ng L−1 TBT-exposed juvenile mysids than in control and DMSO-exposed groups. Levels of 20E were significantly lower at 5 and 10 ng L−1 TBT exposures. Additionally, the number of newly hatched juveniles was significantly lower from females previously exposed to 10 ng L−1 TBT. Our results indicate sublethal concentrations of TBT have inhibitory effects on the survival, growth, and production of juveniles. The lower 20E levels could be strongly associated with TBT-triggered inhibition.