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Application of Chitosan in Veterinary Vaccine Production

Albulov, A. I., Frolova, M. A., Grin, A. V., Kovaleva, E. I., Melnik, N. V., Krasochko, P. A.
Applied biochemistry and microbiology 2018 v.54 no.5 pp. 518-521
adjuvants, animals, biological production, chitosan, drying, hemagglutination tests, immunogenicity, inactivated vaccines, infectious bovine rhinotracheitis, sodium chloride, solubility, solvents, succinic acid, surveys
Probability-based surveys of the application of chitosan succinate as an adjuvant added to the vaccine against animal necrobacteriosis have been performed. The addition of 3.7% chitosan succinate (MM 330 kDa) to the standard-dose vaccine formulation could contribute to improvement of the vaccine immunogenicity, as measured by the hemagglutination test (HA): 1 : 512 as compared to 1 : 128 in the control range. The use of 0.5-% chitosan succinate solution as a protective medium for drying in the production of a dried inactivated vaccine against infectious bovine rhinotracheitis could ensure the preservation of the biopreparation’s high immunogenic activity. Chitosan succinate was shown to have quite good solubility in water, allowing the possible use of saline solution to reconstitute the vaccine into the suspension for injection and to abandon expensive solvents.