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Molecular characterization of triosephosphate isomerase from Echinococcus granulosus

Author:
Wu, Maodi, Yan, Min, Xu, Jing, Yin, Xiaoxiao, Dong, Xiaowei, Wang, Ning, Gu, Xiaobin, Xie, Yue, Lai, Weimin, Jing, Bo, Peng, Xuerong, Yang, Guangyou
Source:
Parasitology research 2018 v.117 no.10 pp. 3169-3176
ISSN:
0932-0113
Subject:
Echinococcus granulosus, Taenia hydatigena, Taenia multiceps, Western blotting, adults, antigens, bioinformatics, blood serum, echinococcosis, energy, enzyme-linked immunosorbent assay, glycolysis, immunogenicity, larvae, neck, protoscoleces, sheep, tissue distribution, triose-phosphate isomerase, zoonoses, China
Abstract:
Cystic echinococcosis (CE) is a zoonosis that can be caused by the larvae of Echinococcus granulosus; this disease occurs worldwide and is highly endemic in China. E. granulosus can produce energy by glycolysis as well as both aerobic and anaerobic respirations. Triosephosphate isomerase is a glycolytic enzyme present in a wide range of organisms and plays an important role in glycolysis. However, there has been little research on triosephosphate isomerase from E. granulosus (Eg-TIM). Here, we present a bioinformatic characterization and the experimentally determined tissue distribution characteristics of Eg-TIM. We also explored its potential value for diagnosing CE in sheep using indirect enzyme-linked immunosorbent assay (ELISA). Native Eg-TIM was located in the neck and hooks of protoscoleces (PSCs), as well as the tegument and parenchyma tissue of adult worms. The entire germinal layer was also Eg-TIM positive. Western blots showed that recombinant Eg-TIM (rEg-TIM) reacts with positive serum from sheep and had good immunogenicity. Indirect ELISA exhibited low specificity (53.6%) and low sensitivity (87.5%) and cross-reacted with both Taenia multiceps and Taenia hydatigena. Our results suggest that TIM may take part in the growth and development of E. granulosus. Furthermore, we determined that rEg-TIM is not a suitable serodiagnostic antigen for CE in sheep.
Agid:
6139132