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Molecular characterization of triosephosphate isomerase from Echinococcus granulosus

Wu, Maodi, Yan, Min, Xu, Jing, Yin, Xiaoxiao, Dong, Xiaowei, Wang, Ning, Gu, Xiaobin, Xie, Yue, Lai, Weimin, Jing, Bo, Peng, Xuerong, Yang, Guangyou
Parasitology research 2018 v.117 no.10 pp. 3169-3176
Echinococcus granulosus, Taenia hydatigena, Taenia multiceps, Western blotting, adults, antigens, bioinformatics, blood serum, echinococcosis, energy, enzyme-linked immunosorbent assay, glycolysis, immunogenicity, larvae, neck, protoscoleces, sheep, tissue distribution, triose-phosphate isomerase, zoonoses, China
Cystic echinococcosis (CE) is a zoonosis that can be caused by the larvae of Echinococcus granulosus; this disease occurs worldwide and is highly endemic in China. E. granulosus can produce energy by glycolysis as well as both aerobic and anaerobic respirations. Triosephosphate isomerase is a glycolytic enzyme present in a wide range of organisms and plays an important role in glycolysis. However, there has been little research on triosephosphate isomerase from E. granulosus (Eg-TIM). Here, we present a bioinformatic characterization and the experimentally determined tissue distribution characteristics of Eg-TIM. We also explored its potential value for diagnosing CE in sheep using indirect enzyme-linked immunosorbent assay (ELISA). Native Eg-TIM was located in the neck and hooks of protoscoleces (PSCs), as well as the tegument and parenchyma tissue of adult worms. The entire germinal layer was also Eg-TIM positive. Western blots showed that recombinant Eg-TIM (rEg-TIM) reacts with positive serum from sheep and had good immunogenicity. Indirect ELISA exhibited low specificity (53.6%) and low sensitivity (87.5%) and cross-reacted with both Taenia multiceps and Taenia hydatigena. Our results suggest that TIM may take part in the growth and development of E. granulosus. Furthermore, we determined that rEg-TIM is not a suitable serodiagnostic antigen for CE in sheep.