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Filarial nematode (order: Spirurida) surveillance in urban habitats, in the city of Pécs (Hungary)

Kurucz, Kornélia, Kiss, Vivien, Zana, Brigitta, Jakab, Ferenc, Kemenesi, Gábor
Parasitology research 2018 v.117 no.10 pp. 3355-3360
Aedes vexans, Coquillettidia, DNA, Dirofilaria immitis, Dirofilaria repens, Setaria tundra, adults, females, habitats, humans, invasive species, monitoring, mosquito control, parasites, polymerase chain reaction, sequence analysis, surveys, Hungary
As part of the seasonal mosquito control activities in the city of Pécs (Baranya County, Hungary), a total of 1123 adult female mosquitoes belonging to 18 species (including the invasive species Aedes koreicus) were collected from human-inhabited areas, using CO₂-baited traps, during two consecutive years. To survey the presence and prevalence of filarial parasites in these mosquitoes, we performed a molecular survey for filarial DNA, attempted by PCR using generic primers (COI), and followed by DNA sequencing. Filaroid nematode DNA was detected in 4% of investigated mosquito pools. Out of 410 pools, 9 pools of mosquitoes were positive for Dirofilaria repens (Aedes vexans, Aedes koreicus, Coquillettidia richiardii), and/or Dirofilaria immitis (Ae. vexans, Cq. richiardii), and further 8 pools were positive for Setaria tundra (Ae. vexans, Cq. richiardii). Our study provides novel insight for prevalence of filaroid nematodes in mosquitoes occurring in close proximity to humans, thereby highlights the possible human and veterinary health importance of these mosquito species, including the recently introduced invasive mosquito Ae. koreicus.