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Carbon dioxide induces minor antioxidant responses in Eucalyptus urophylla chloroplasts

Baldassi, Amanda Cristina, dos Santos, Bruna Marques, Balbuena, Tiago Santana
Trees 2018 v.32 no.5 pp. 1481-1485
C3 plants, Eucalyptus urophylla, antioxidant activity, ascorbate peroxidase, carbon dioxide, carbon dioxide fixation, chloroplast proteins, chloroplasts, climate change, hydrogen peroxide, photosynthesis, proteomics
KEY MESSAGE: Minor effect on the chloroplast antioxidant proteins was detected in Eucalyptus urophylla cultivated in high-CO₂ atmosphere. Global climate change can significantly alter plant cell metabolism. A higher atmospheric CO₂ scenario may be beneficial for C3 plants through the stimulation of photosynthesis. This predicted increase in the rate of carbon assimilation may also increase the expression of enzymes involved in the antioxidant cellular defense. Here, we studied the responses of the chloroplastic antioxidant system of Eucalyptus urophylla plants cultivated in a high-CO₂ condition. Plants exposed to a high concentration (980 ppm) of CO₂ showed an increase in the H₂O₂ concentration and MDA content in relation to those cultivated at 410 and 680 ppm. With the discovery proteomics approach used herein, we identified 19 chloroplastic antioxidant proteoforms and pinpointed differentially regulated isoforms of an ascorbate peroxidase and a superoxidase dismutase upon cultivation in a high-CO₂ atmosphere. Our data indicate that the CO₂ stimulus induces only minor changes in the antioxidant metabolism of E. urophylla chloroplasts.