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Co-infection and disease severity of Ohio Maize dwarf mosaic virus and Maize chlorotic dwarf virus strains

Katia Morales, Jose Luis Zambrano, Lucy R. Stewart
Plant disease 2014 v.98 no.12 pp. 1661-1665
Maize chlorotic dwarf virus, Maize dwarf mosaic virus, Zea mays, corn, disease severity, greenhouse experimentation, growth and development, mixed infection, plant growth, reverse transcriptase polymerase chain reaction, signs and symptoms (plants), viruses, Ohio
Two major maize viruses have been reported in the United States: Maize dwarf mosaic virus (MDMV) and Maize chlorotic dwarf virus (MCDV). These viruses co-occur in regions where maize is grown such that co-infections are likely. Co-infection of different strains of MCDV is also observed frequently, and a synergistic enhancement of symptoms in co-infected plants was previously reported. Here we examined the impact of co-infections of two strains of MCDV (MCDV-S and MCDV-M1, severe and mild), and co-infections of MCDV and MDMV in ‘Spirit’ maize in greenhouse experiments. Quantitative plant growth and development parameters were measured and virus accumulation was measured by RT-qPCR. Based on our results, we conclude that virus symptoms are enhanced and plants show less recovery over time in co-infections of MDMV and MCDV-S compared to single infections with either virus. In contrast, MDMV co-infections with MCDV-M1 did not show symptom enhancement for either virus, and co-infection of MCDV-M1 and MCDV-S results in symptoms intermediate between the two single infections.